METHODS TO FABRICATE LIVING CELL VESSELS OF NON-UNIFORM AND SPATIALLY HETEROGENOUS ARCHITECTURE

原文（中文）

A method for fabricating an in vitro vessel. The method may include forming a substrate that defines a microfluidic passage therein including variations in size and shape along a length of the microfluidic passage, positioning the substrate in a vertical orientation whereby an acute angle is formed between a longitudinal direction of extension of the microfluidic passage and a direction of gravity and culturing a plurality of first cells in the microfluidic passage while the substrate is disposed in the vertical orientation whereby an annular layer of the plurality of first cells is formed in the microfluidic passage. The layer of the plurality of first cells may define a lumen extending longitudinally through the microfluidic passage. The lumen includes a complex vascular architecture comprising a bifurcation, tortuosity, a stenosis, an aneurysm, or combinations thereof.