SYSTEM FOR NON-PERTURBATIVE SAMPLING OF SAMPLE-VOLUME-LIMITED BIOREACTORS

原文（中文）

A non-perturbative sampling system includes a bioreactor comprising a chamber containing media with cells; an input tube for delivering nutrient-laden media to the chamber; and an output tube for withdrawing a sample from the chamber; an input pump configured to deliver the nutrient-laden media at an inflow rate; an output pump configured to withdraw the sample at an outflow rate; and a controller configured to operate the input and output pumps to regulate the inflow and outflow rates, respectively, such that the bioreactor is a variable-volume bioreactor in which an instantaneous volume of media either varies continuously with time or is held constant, and operable in sample accumulation phase, sample storage phase, sample withdrawal phase, or volume restoration phase, to maintain the quantity of nutrients per cell within the media in the chamber unchanged. The sample withdrawal phase either follows the sample accumulation phase or precedes the volume restoration phase.