MICROFLUIDIC ELECTROPHORESIS-MEDIATED CHARACTERIZATION OF PLASMID DNA ISOFORMS

原文（中文）

Methods of assessing double-stranded plasmid DNA isoforms to determine a proportion of supercoiled plasmid DNA compared to linear plasmid DNA, and/or open circle plasmid DNA are provided according to aspects of the present disclosure, which include: preferentially labelling double-stranded plasmid DNA; flowing the labeled double-stranded plasmid DNA through a polymeric separation medium in a microchannel into a detection region; detecting the detectable label to determine: a) an amount of time taken by the labeled plasmid DNA isoforms to flow through the polymeric separation medium in the microchannel into the detection region, and/or b) strength of the signal of the detectable label in the detection region; and comparing a) and/or b) to a reference standard thereby determining a proportion of supercoiled plasmid DNA compared to linear plasmid DNA, and/or open circle plasmid DNA in the fluid sample comprising plasmid DNA.