IMAGE SCANNING MICROSCOPE AND METHOD

原文（中文）

An image scanning microscope includes an excitation unit that generates excitation light according to an excitation modality, and an objective lens directed at a sample space to direct the excitation light and to receive the detection light. A scanning unit of the image scanning microscope is arranged along a beam path between the excitation unit and the objective lens to selectively direct the excitation light. The image scanning microscope includes a detection arrangement including a spectral encoding element to change the spatial distribution of the intensity of the detection light and an array detector. A main beam splitter of the image scanning microscope directs the excitation light into the objective lens, and directs the detection light. The image scanning microscope includes a control unit to control the excitation unit to set the excitation modality, and to determine a spatial distribution of a concentration of two different fluorophore species.