Display substrate, preparation method thereof and display device

The invention provides a display substrate, a preparation method thereof and a display device. The display substrate comprises a pixel area and a stretching hole area located on the outer side of the pixel area, the stretching hole area comprises a hole area and a partition area surrounding the hole area, and the partition area comprises a partition structure layer surrounding the hole area, a packaging structure layer arranged on the partition structure layer and a sensing structure layer arranged on the packaging structure layer. The partition area further comprises a first organic dielectric layer located between the packaging structure layer and the sensing structure layer, and the side, close to the hole area, of the first organic dielectric layer covers the partition structure layer and the side face, close to the side of the hole area, of the packaging structure layer.