Color film substrate, manufacturing method thereof and display panel

The invention discloses a color film substrate, a manufacturing method thereof and a display panel, and belongs to the technical field of display. The color film substrate comprises a plurality of sub-pixel regions distributed in an array and spacer regions located among the plurality of sub-pixel regions, and the color film substrate comprises a substrate, a plurality of sub-pixel regions and a plurality of sub-pixel regions, the color film layer is positioned on one side of the substrate; and the conductive layer is located in the spacer region and located on the side, away from the color film layer, of the substrate. According to the display panel, electric field interference on the sub-pixel areas can be reduced, liquid crystal deflection in the display panel is accelerated, and therefore the response time of liquid crystals is shortened.