METHODS AND SYSTEMS FOR IMAGING INTERACTIONS BETWEEN PARTICLES AND FRAGMENTS
METHODS AND SYSTEMS FOR IMAGING INTERACTIONS BETWEEN PARTICLES AND FRAGMENTS
Methods and systems for imaging interactions between particles and fragments are provided. A method includes applying a template to one or more images of a sample comprising a particle and a fragment. The template comprises a three-dimensional representation of the particle at a resolution of higher than about ⅛ reciprocal Angstroms and is produced by data independent of data provided in the one or more images. The fragment is not represented in the template. A similarity image is produced comprising a pixel-wise representation of a distance metric between the template and the one or more images. The distance metric enables detection of at least a portion of the particle or fragment. A threshold is applied to the similarity image to distinguish positive detections from noise and a representation of a volume as a function of the positive detections is produced, representing an interaction between the particle and the fragment.