INTERGRATED MICRO-DROPLET CHIP AND MICRODROPLET MULTI-INDEX DETECTION METHOD THEREFOR

원문 (중국어)

The present application provides an integrated droplet chip and a droplet multi-index detection method therefor and belongs to the technical field of digital PCR analyzers. The chip comprises a chip body, the chip body being constructed with at least one set of one-to-many mixing and reaction structures, a droplet generating structure and a fluorescence detection area, and each of the one-to-many mixing and reaction structures comprises: a primary reaction chamber, used for performing primary amplification, so that a detection sample with a first concentration in the primary system is amplified to form a detection sample with a second concentration, wherein the second concentration is higher than the first concentration; a plurality of sampling chambers; and the plurality of sampling chambers are controllably connected to the same primary reaction chamber at the same time; a plurality of secondary reaction chambers, which are controllably connected to the plurality of sampling chambers in a one-to-one correspondence, are used to store the droplets generated by the detection sample of the second concentration and the secondary system at the droplet generating structure and perform secondary amplification. The present application effectively increases the concentration of the detection sample. The detection sample having the increased concentration is divided into more detection samples to ensure that each detection sample has a relatively high concentration, thereby achieving multi-index detection of a low-concentration sample, ensuring the sensitivity of sample detection, and improving the accuracy of detection results.