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기록

IMAGING FLOW CYTOMETRY-BASED HIGH-THROUGHPUT DRUG SCREENING METHOD

발명심사 중
9청구항 · 1 독립항
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개요

발명자

Jingjing ZHAO; Lin DU

IPC 분류

G1N 15/1434G1N 1/30G1N 15/1G1N 15/10G1N 15/1404G1N 15/1433G1N 33/58

CPC 분류

G1N15/1434G1N1/30G1N15/1G1N15/1404G1N15/1433G1N33/582G1N2015/1006G1N2015/144G1N2500/10

An imaging flow cytometry-based high-throughput drug screening method, comprising: cell incubation, cell staining, acquiring single-cell images by using a flow cytometer, and image extraction and analysis, which combines the high-throughput advantages of flow cytometry and the imaging capability of a microscope, so that multi-channel single-cell images can be generated in a high throughput manner, thereby implementing acquisition of fluorescent images and unmarked images of single cells at a throughput of 102-105 cells per second, which can be used for cell phenotype drug screening and improving the screening efficiency by a factor of 102-104.

원문 (중국어)

An imaging flow cytometry-based high-throughput drug screening method, comprising: cell incubation, cell staining, acquiring single-cell images by using a flow cytometer, and image extraction and analysis, which combines the high-throughput advantages of flow cytometry and the imaging capability of a microscope, so that multi-channel single-cell images can be generated in a high throughput manner, thereby implementing acquisition of fluorescent images and unmarked images of single cells at a throughput of 102-105 cells per second, which can be used for cell phenotype drug screening and improving the screening efficiency by a factor of 102-104.