Digital microfluidic nucleic acid detection chip, detection method and detection device

The invention relates to a digital microfluidic nucleic acid detection chip (40), a detection method and a detection device. The digital microfluidic nucleic acid detection chip (40) comprises a first substrate (1) and a second substrate (2) which are arranged in a box-to-box manner, a cavity formed between the first substrate (1) and the second substrate (2) comprises a functional region (AC), and the functional region (AC) is configured to perform nucleic acid detection processing on a liquid drop (4) to be detected and obtain a hybridization developing signal for indicating whether a target gene exists in the liquid drop (4) to be detected or not; the first substrate (1) at least comprises a plurality of driving units (3), the plurality of driving units (3) are configured to drive the to-be-measured liquid drop (4) to move, the volume of the to-be-measured liquid drop (4) is 10 microliters to 200 microliters, and the size of each driving unit (3) in the moving direction of the to-be-measured liquid drop (4) is 2mm to 100mm.