Display substrate, preparation method thereof and display panel

The invention provides a display substrate, a manufacturing method of the display substrate and a display panel, belongs to the technical field of display, and can solve the problem that the characteristics of an existing display substrate are abnormal due to the fact that an anti-reflection layer is arranged below a metal signal line of the existing display substrate. The display substrate of the present disclosure comprises: a substrate; the first conductive layer at least comprises a source electrode, a drain electrode and a first signal line; the semiconductor layer comprises an active layer and is electrically connected with a source electrode and a drain electrode of the active layer; the two opposite side faces, in the thickness direction of the active layer, of the active layer are not in contact with the first anti-reflection layer; the first anti-reflection layer comprises a first anti-reflection pattern, the orthographic projection of the first anti-reflection pattern on the substrate is at least partially overlapped with the orthographic projection of the first signal line on the substrate, and the first anti-reflection pattern is configured to reduce the reflectivity of the first signal line. On the premise that the characteristics of the display substrate are not affected, the problem of data line number line reflection reduction is solved.