Display panel, manufacturing method of display panel and display device

The invention discloses a display panel, a manufacturing method of the display panel and a display device, and belongs to the technical field of display. The display panel comprises a substrate, a pixel defining layer, a plurality of pixel structures, a plurality of isolation structures and a plurality of supporting structures, the pixel defining layer is provided with a first opening and a second opening, and the supporting structures are located on the side, away from the substrate, of the pixel defining layer. The supporting structure can comprise a supporting base and a supporting pad which are stacked in the direction away from the substrate, and the orthographic projection of the supporting pad on the substrate is located in the orthographic projection of the supporting base on the substrate. The stability of the supporting structure is guaranteed through the supporting base with the large size, and the contact area between the supporting pad and the mask is reduced through the supporting pad with the small size, so that the probability that the mask scratches the supporting pad is reduced.