CHEMICAL SENSORS EMPLOYING pH-SENSITIVE APTAMERS

原文（中文）

Described herein are sensors for detecting an analyte of interest in a sample. These sensors can comprise a potentiometric sensor comprising a surface functionalized with a pH-sensitive aptamer switch that specifically binds the analyte of interest, wherein the pH-sensitive aptamer switch is operatively coupled to the potentiometric sensor such that binding of the analyte of interest by the pH-sensitive aptamer switch induces a measurable change in the potentiometric sensor; and an auxiliary electrode in proximity to the surface, wherein the electrode is configured to alter a pH of a microenvironment in contact with the surface, thereby reversibly shuttling the pH-sensitive aptamer switch between a first state wherein it specifically binds the analyte of interest and a second state wherein it does not specifically bind the analyte of interest.