一种无抗质粒生产菌株及其应用
本公开内容提供一种无抗质粒的生产菌株,所述生产菌株为PIR菌株的基因编辑菌株,命名为PIR1‑WN::0636或PIR1‑PR::0636,所述生产菌株含有编码毒素蛋白的核苷酸序列和无抗质粒,所述无抗质粒含有编码抗毒素蛋白的核苷酸序列;无抗质粒优选复制子DNA元件为R6K‑γ。本发明的生产菌株毒素蛋白基因能够稳定传代,经过诱导具致死性可用于质粒筛选;本发明中无抗质粒转化至PIR1‑WN::0636菌株阳性率80%以上,且可以实现高超螺旋比例的质粒稳定生产。
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