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Original name record: 福建省农业科学院农业质量标准与检测技术研究所

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№ CN121320591AInvention
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基于一管法RPA-CRISPR/Cas12a的副溶血弧菌快速检测试剂盒及检测方法

本发明涉及一种基于一管法RPA‑CRISPR/Cas12a的副溶血弧菌快速检测试剂盒及检测方法。以副溶血弧菌ToxR基因为靶标，设计了特异性RPA引物和CRISPR crRNA。所建立的一管法RPA‑CRISPR/Cas12a检测体系，有效避免了开盖过程导致的气溶胶污染风险。本发明方法特异性强，与创伤弧菌、溶藻弧菌、霍乱弧菌、哈维氏弧菌、鳗弧菌等近缘弧菌，以及铜绿假单胞菌、杀香鱼假单胞菌等其他水产病原菌均无交叉反应；灵敏度高，对基因组DNA的最低检测限低至1 pg/μL；检测迅速，核酸扩增与荧光检测全过程仅需约30分钟。该试剂盒及方法无需复杂仪器设备，操作简单，适合于基层对水产品样本进行现场快速检测，在副溶血弧菌的早期筛查、流行病学调查及综合防控中均具有重要应用价值。

❖福建省农业科学院农业质量标准与检测技术研究所刘兰英; 吕新; 罗土炎; 饶秋华; 刘洋; 黄薇; 任丽花§ 4/10 ClaimsC12Q 1/689 (2018.01), C12Q 1/6844 (2018.01), C12N 15/11 (2006.01) +1