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№ CN122979529AInvention

Unknown

传染性造血器官坏死病毒全预混实时荧光检测试剂盒

本发明提供传染性造血器官坏死病毒全预混实时荧光检测试剂盒，属于生物检测技术领域。该试剂盒包括RT‑qPCR全预混检测混合液，该混合液包括上、下游茎环引物、检测探针和酶；上游茎环引物包含茎部和环部，茎部由其自身功能域1与功能域3互补配对而成，环部为其自身功能域2，上游茎环引物功能域3的3’端连接其自身功能域4；下游茎环引物包含茎部和环部，茎部由其自身功能域1与功能域3互补配对而成，环部为其自身功能域2，下游茎环引物功能域 3 的 3’端连接其自身功能域 4；检测探针的核苷酸序列如SEQ ID No:4所示。本发明试剂盒均一稳定，能够快速、高灵敏度、高特异性地检测传染性造血器官坏死病毒，污染易控。

❖南京农业大学; 深圳海关动植物检验检疫技术中心孙洁; 姜平; 白娟; 朱崧琪; 王津津; 佟宗轩; 火兴民; 蔡赟; 魏玉海Oct 27, 2026§ 2/5 ClaimsC12Q 1/70 (2006.01), C12Q 1/6851 (2018.01), C12N 15/11 (2006.01) +1

№ CN122918164AInvention

Unknown

一种小麦线条花叶病毒检疫除害处理与灭活效果评价方法

本发明涉及植物检疫与植物病毒学技术领域，尤其涉及一种小麦线条花叶病毒检疫除害处理与灭活效果评价方法，包括将携带小麦线条花叶病毒的样品进行热处理；对热处理后的样品进行灭活效果双重评价，包括生物接种测定和RT‑PCR分子生物学检测，两者平行进行、互不依赖；生物接种测定为将热处理后样品汁液摩擦接种于指示植物叶片，观察是否出现典型症状；RT‑PCR检测为提取总RNA进行扩增，检测是否存在WSMV核酸；当生物接种无症状且RT‑PCR无扩增条带时，判定病毒已完全灭活。本发明处理时间仅为分钟级，且对种子活力影响小，填补了小麦等粮食物料中小麦线条花叶病毒检疫处理技术的空白。

❖深圳海关动植物检验检疫技术中心余道坚; 郑耘; 李萍; 杨东; 赖文涛; 冼嘉恒Oct 16, 2026§ 1/10 ClaimsC12Q 1/22 (2006.01), C12Q 1/70 (2006.01), C12Q 1/686 (2018.01) +5