一种用于降低抗体磺酸化水平的细胞培养方法
本发明涉及细胞工程领域,特别涉及一种用于降低抗体磺酸化水平的细胞培养方法。在宿主细胞以预设密度接种至反应瓶中培养,并在多个预设时间点以第一补料培养基进行补料,在培养的第5日将培养温度降低至30℃~32℃中的任一值,在培养第4天和第6天分别向反应瓶中加入氯酸钠,氯酸钠的总添加浓度为35mM~45mM中的任一值。通过氯酸钠抑制磺酸化的关键原料活性硫酸基供体3'‑磷酸腺苷‑5'‑磷酸硫酸的生成,从而阻断抗体磺酸化路径,降低产物中发生磺酸化修饰的比例。
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