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№ CN122979401AInvention

Unknown

一种检测补体依赖性细胞毒性的试剂盒、方法及应用

本申请公开了一种检测补体依赖性细胞毒性的试剂盒、方法及应用，方法用于非诊断目的检测AQP4‑IgG阳性血清补体依赖性细胞毒性，包括：获得瞬时表达AQP4且非融合独立表达ZsGreen荧光蛋白的HEK293T细胞；将HEK293T‑AQP4细胞接种于96孔板中，再培养至细胞汇合；洗涤后，孵育30分钟；向细胞悬液中加入5 μL 7‑氨基放线菌素D进行染色，于室温下避光孵育10分钟；采用流式细胞仪检测存活靶细胞的百分比。本申请通过表达AQP4与ZsGreen的HEK293T细胞作为靶细胞，结合7‑AAD死活细胞染色和流式细胞术，实现了对血清CDC活性的高通量、客观、定量检测。

❖天津医科大学总医院杨春生; 蒋薇; 徐晓娜; 李晓; 丁洁; 杜雨芃; 薛慧慧Oct 27, 2026§ 3/7 ClaimsC12Q 1/02 (2006.01), G01N 33/68 (2006.01), G01N 33/569 (2006.01) +6

№ CN122991447AInvention

Unknown

一种辅助老年人用药与健康管理的智能提醒方法及系统

本申请涉及一种辅助老年人用药与健康管理的智能提醒方法及系统。所述方法包括：对基础感知数据无监督学习生成初始反应与本底环境特征，结合用药处方指令生成个体化反应延迟基准线、环境声学本底噪声频谱和服药时间窗；基于多维传感器数据进行自适应多模态提醒调度，生成最优策略组合向量控制提醒设备组；通过隐马尔可夫模型研判用户动作状态，异常时进行二次提醒与认知核验，低分则下发趋光引导指令；完成服药后进行弱网因果同步并向局域网节点广播数据指令。采用本方法能够精准适配老年人用药习惯，提升用药提醒成功率与健康管理可靠性。

❖天津医科大学总医院周丽微; 仝家瑜; 张雅静Oct 27, 2026§ 4/9 ClaimsG16H 20/10 (2018.01), G16H 40/67 (2018.01), G16H 50/70 (2018.01) +3