一种能高效捕获短片段的单链DNA文库构建方法及应用
本发明涉及一种单链DNA文库的构建方法及其应用。具体地,通过polyC加尾后进行线性扩增,提高了连接接头的效率,并且在扩增过程中按一定比例加入生物素标记的dCTP,最大程度地保留了文库中的短片段。该方法适用于较低的起始量,对于片段受损、降解严重的稀有样本也可以进行文库构建。
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