R环感测器
本发明涉及检测细胞中R环的方法,其包括培养表达DNA:RNA杂交体结合的融合蛋白的分离的细胞,所述蛋白包含:(a)两个或更多个杂交体结合结构域(HBD);和(b)可检测的报告结构域,使得所述融合蛋白能结合细胞中的R环。然后,测定所述融合蛋白在所述细胞中的分布。融合蛋白的积累指示细胞中的R环。还提供了DNA:RNA杂交体结合的融合蛋白、编码核酸以及筛选调节R环的化合物的方法。
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