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Original name record: 湖南融健生物科技有限公司

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1 indexed patent records

№ CN121336561AInvention
Active

一种脱除苦瓜种子中黄瓜花叶病毒的方法

本发明涉及种子处理技术领域，具体而言，涉及一种脱除苦瓜种子中黄瓜花叶病毒的方法，包括以下步骤：步骤一，纤维素酶预处理；步骤二，纳米粒子及光照射处理：将步骤一处理后的种子用包裹有光敏剂的纳米粒子溶液、蛋白酶K和十二烷基硫酸钠的混合液浸泡，然后加入过氧化氢溶液浸泡后，用与光敏剂最大吸收波长相匹配的光进行照射，照射完成后清洗；步骤三，干燥：将步骤二清洗后的种子进行干燥处理；步骤四，发芽检测：取部分步骤三得到的种子进行发芽试验，观察并记录种子发芽率；步骤五，RT‑LAMP检测：采用RT‑LAMP方法对步骤四获得的种苗进行黄瓜花叶病毒检测，多重处理，可有效脱除苦瓜种子中的黄瓜花叶病毒。

❖湖南融健生物科技有限公司卿琰; 蒋健晖; 唐昊; 黄志梅; 楚霞; 黄琴§ 1/10 ClaimsA01C 1/00 (2006.01), A01C 1/02 (2006.01), C12Q 1/70 (2006.01) +610