一种百香果遗传转化方法
本发明公开了一种百香果遗传转化方法,属于植物生物技术领域。该方法以百香果幼嫩茎节为外植体,经表面消毒后利用含目的基因表达载体的农杆菌进行侵染和共培养,经含抗生素的筛选培养基愈伤诱导和筛选后,将抗性愈伤转移至分化培养基上分化再生,最后转移至生根培养基生根,获得转基因植株。本发明通过优化培养基配方实现了百香果高效胚性愈伤诱导和稳定再生,操作简单,适用基因型范围广等优势,为百香果基因编辑育种和品种改良提供了关键技术支撑。
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