一种石斛小RNA miR421及其应用
本发明公开了一种石斛小RNA miR421及其应用。miR421在调控植物多糖合成中的应用,所述的miR421的成熟序列如SEQ ID NO.1所示。本发明从铁皮石斛叶片中克隆得到了属于石斛的miR421,miR421作为负调控因子参与了调控石斛多糖合成过程,通过瞬时表达及功能鉴定证实超量表达miR421,转基因石斛的多糖含量降低。因此,miR421在兰科植物的次生代谢产物合成和医药应用领域均有重要的理论指导意义及应用价值。
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