脱保护试剂、试剂盒及其应用
本发明属于化学合成领域,尤其涉及脱保护试剂、试剂盒及其应用,所述脱保护试剂包括以下组分:1‑羟基苯并三唑;有机碱。本发明的脱保护试剂,通过1‑羟基苯并三唑、有机碱的协同作用,实现了温和高效脱保护与关键副反应抑制的有机统一,具体而言,1‑羟基苯并三唑不仅能有效抑制氨基酸消旋化,更通过捕获β‑消除反应中间体,与有机碱共同构建出针对磷酸化氨基酸的保护机制,从而制备获得高纯度、结构准确的复杂磷酸化蛋白/多肽奠定了坚实基础。
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