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Original name record: 深圳市北科生物科技有限公司; 深圳市北科源细胞科技有限公司

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№ CN121320248AInvention
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一种通过iPSC分化诱导获得并激活扩增自然杀伤细胞的方法

本发明公开一种通过iPSC分化诱导获得并激活扩增自然杀伤细胞的方法，涉及干细胞生物学技术领域，该种通过iPSC分化诱导获得并激活扩增自然杀伤细胞的方法包括如下步骤：S1、iPSC细胞准备与接种；S2、iPSC向中胚层分化；S3、中胚层细胞向CD34+生血内皮细胞分化；S4、CD43+/CD45+造血干细胞向iNK细胞分化；S5、iNK细胞成熟；S6、iNK细胞激活扩增；步骤S1中用新鲜E8完全培养基重悬细胞，接种于多孔培养板中；培养过程中使用多种诱导培养基；通过采用多孔培养板并使用E8完全培养基重悬细胞，实现了iPSC的高效接种和初步培养，提高了细胞的生存率和扩增效率；通过使用多种诱导培养基优化了iPSC向中胚层、生血内皮细胞和CD34+造血干细胞的分化过程，提高了分化效率。

❖深圳市北科生物科技有限公司; 深圳市北科源细胞科技有限公司蔡车国; 傅泽钦; 郭艳秋; 胡樾; 王玉霞; 李梦园; 胡隽源§ 14/14 ClaimsC12N 5/0783 (2010.01), C12N 5/074 (2010.01), C12N 5/10 (2006.01)