具有发夹样插入物的RNA载体
本公开涉及一种具有外源RNA区段的病毒载体,所述外源RNA区段具有发夹样结构,所述发夹样结构例如具有由一个或多个非碱基配对区域分开的两个或更多个碱基配对区域。所述外源RNA区段可具有在指定范围内的二级结构、最小自由能、平均位置熵或其他属性,或具有与参考野生型病毒的一个或多个发夹样结构相似的值。在一些示例中,所述病毒载体是活减毒疫苗。在一些示例中,所述病毒载体下调宿主植物中的易感基因。
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