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№ CN122918255AInvention

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应用LFD-RPA技术检测葡萄白腐病菌的引物对、探针、试剂盒及其应用

本发明属于生物技术领域，具体涉及到一种应用重组酶聚合酶扩增侧流层技术(LFD‑RPA)快速检测葡萄白腐病菌的引物对、探针、试剂盒及其应用。本发明针对葡萄白腐病的致病菌葡萄白腐病菌（Coniella vitis）设计了特定的引物对、探针，并与LFD技术相结合，实现了对葡萄白腐病菌便捷、高度特异性和高灵敏度的RPA检测，灵敏度可达10ng/μL，在葡萄白腐病菌的早期诊断、田间诊断和辅助鉴定方面有很高的应用价值。

❖安徽省农业科学院植物保护与农产品质量安全研究所; 安徽省农业科学院园艺研究所杨雪; 孙其宝; 周军永; 陆丽娟; 徐会永; 谷春艳; 潘锐; 臧昊昱Oct 16, 2026§ 5/10 ClaimsC12Q 1/6895 (2018.01), C12Q 1/6844 (2018.01), C12N 15/11 (2006.01) +120