一种前列腺癌检测试剂盒、检测体系、检测方法及应用
本发明提供一种前列腺癌检测试剂盒、检测体系、检测方法及应用,所述试剂盒同时检测尿液样本中PCA3和PSA的Ct值,即Ct和Ct;所述试剂盒包括针对PCA3基因的第一特异性扩增引物和探针组,以及,针对的PSA基因的第二特异性扩增引物和探针组;所述试剂盒根据ΔCt值判读检测结果,其中,ΔCt=Ct-Ct。本发明的检测试剂盒及检测体系操作流程简单,耗时较短,结果判读方法简便、结果重复性与特异性好,与临床评价指标符合率高。
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