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Original name record: 厦门飞朔生物技术有限公司

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1 indexed patent records

№ CN121344197AInvention
Active

一种基于数字PCR单管检测ESR1基因耐药突变位点的引物探针组合及其应用

本发明公开了一种基于数字PCR单管检测ESR1基因耐药突变位点的引物探针组合及其应用，属于分子检测技术领域，其中引物的序列如SEQ ID NO.1‑2所示，探针的序列如SEQ ID NO.3‑4所示，两个探针的5’端标记不同的荧光基团。本发明利用上述引物探针组合实现了单管同时对乳腺癌中常见的ESR1基因L536H、L536Q、L536R、L536P、Y537S、Y537C、Y537N、Y537D、D538G共9个耐药相关突变位点的高灵敏度且快速的检测，大大提高了ESR1基因检测效率，并降低了检测成本，以便指导乳腺癌的内分泌治疗。

❖厦门飞朔生物技术有限公司陈琴; 陈志宏; 吴水英; 陈琰§ 3/10 ClaimsC12Q 1/6886 (2018.01), C12Q 1/6858 (2018.01), C12N 15/11 (2006.01)10