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一种用于提取植物组织细胞核的试剂盒

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7Claims · 3 independent
§ Ⅰ

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IPC Classification

C12Q 1/6806 (2018.01)C12N 15/10 (2006.01)

本发明公开了一种用于提取植物组织细胞核的试剂盒,属于生物技术领域。所述试剂盒包括细胞核裂解液和细胞核清洗液,其中,所述细胞核裂解液由蔗糖、pH=7~8的Tris‑HCl、NaCl、MgCl、表面活性剂、DTT、RNase inhibitor和亚精胺利用无核酸酶水配制而成;所述细胞核清洗液由蔗糖、pH=7~8的Tris‑HCl、NaCl、MgCl2、DTT和RNase inhibitor和亚精胺利用无核酸酶水配制而成。本发明的试剂盒适用于红豆杉茎新鲜组织的细胞核提取,得到的细胞核核膜完整、基因表达稳定,纯度高杂质少,可用于单细胞测序。

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