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一种基因工程化马克斯克鲁维酵母、构建方法与应用

InventionUnknown
10Claims · 5 independent
§ Ⅰ

Dossier Overview

Inventor

李俊; 李雨真; 王越; 张舒玥; 安云帆

Filing Date

Jul 16, 2026

Publication Date

Oct 27, 2026

IPC Classification

C12N 15/81 (2006.01)C12N 15/90 (2006.01)C12N 15/53 (2006.01)C12N 15/54 (2006.01)C12N 1/19 (2006.01)C12P 5/02 (2006.01)A61K 8/9728 (2017.01)A61Q 19/08 (2006.01)A61Q 19/00 (2006.01)A61Q 17/04 (2006.01)C12R 1/645 (2006.01)

本发明公开了一种基因工程化马克斯克鲁维酵母的构建方法、菌株与应用。其是将经过密码子优化后的外源基因tHMG基因、Y215CrtBY基因整合至出发菌株的ADH2位点,将经过密码子优化后的外源基因PrCrtE基因、Y215CrtI基因整合至出发菌株的GPD1位点。本发明提供的基因工程菌Y215L能高效发酵生产番茄红素。基因工程菌Y215L在促进成纤维细胞增殖、清除自由基、抗炎、促进成纤维细胞ECM基质分泌能力、促进成纤维细胞抗衰老相关mRNA表达方面也具有工业应用价值。

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