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SND1和NDUFB2双内参基因在沼水蛙肝脏组织荧光定量PCR中的应用
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4Claims · 1 independent
§ Ⅰ
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Inventor
李芳; 洪美玲; 胡明; 王莎; 李荣荣; 艾小琪; 徐彤; 刘思颖
Filing Date
Sep 17, 2026
Publication Date
Oct 27, 2026
IPC Classification
C12Q 1/6888 (2018.01)C12Q 1/6851 (2018.01)C12N 15/11 (2006.01)
本发明公开了SND1和NDUFB2双内参基因在沼水蛙肝脏组织荧光定量PCR中的应用,尤其是SND1和NDUFB2作为双内参基因在亚砷酸钠暴露条件下利用荧光定量PCR检测沼水蛙肝脏组织目标基因表达中的应用,所述SND1的核苷酸序列如SEQ ID NO.1所示,所述NDUFB2的核苷酸序列如SEQ ID NO .2所示,所述沼水蛙肝脏组织目标基因为超氧化物歧化酶1基因,SND1基因和NDUFB2基因在亚砷酸钠暴露条件下沼水蛙肝脏样品中表达稳定,适合作为内参基因组合用于目标基因表达量校正。
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